Generator

Part:BBa_K4475004

Designed by: Andrew Taylor   Group: iGEM22_FSU   (2022-07-18)


CTB1 Constitutive Weak Protein Generator Yeast

Construct designed for weak expression of the fungal enzyme CTB1 in saccharomyces cerevisiae, utilizing the Yeast STE5 Promoter and CYC100 Terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 93
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4133
    Illegal BamHI site found at 4042
    Illegal BamHI site found at 6215
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 93
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 93
    Illegal NgoMIV site found at 6722
    Illegal AgeI site found at 4796
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None